2 edition of Ganglioside composition of normal and mutant mouse embryos found in the catalog.
Ganglioside composition of normal and mutant mouse embryos
Joseph Douglass Bouvier
Written in English
|Statement||by Joseph Douglass Bouvier.|
|Contributions||Boston College. Dept. of Biology.|
|The Physical Object|
|Pagination||50 leaves :|
|Number of Pages||50|
The major GSLs expressed in wild-type (WT) 3 dpf embryos are monohexosylceramide and GM3. Ganglioside expression diversifies during normal development, such that by 5 dpf more complex and multiply sialylated GSLs are detected (Fig. 9). The st3gal5 morphant embryos exhibited reduced expression of GM3 and of the more complex GSLs at 3 and 5 dpf. The ganglioside composition of these regions was the same as that of the whole embryo, with GM3 and GD3 comprising about 75% of the total ganglioside distribution. Moreover, N-acetylneuraminic acid was the only sialic acid species detectable in the E and the E embryos.
Neutral glycolipid abnormalities in at-complex mutant mouse embryo. Biochemical Genetics , 30 (), DOI: /BF Jukka Reivinen, Harry Holthöfer, Aaro Miettinen. A cell-type specific ganglioside of glomerular podocytes in rat kidney: An O-acetylated GD3. In contrast, the ceramide moieties of gangliosides in EB cells were clearly much more heterogeneous. Typically for each of the assigned ganglioside signal clusters, its fatty acyl content was composed of C16 and C16 with an extra OH, C22, C, and C24, among the most abundant components, assuming that each carried the same d sphingosine base.
However, when embryos were stained with acridine orange, which is widely used to detect dying cells during embryonic development in zebrafish and other animals (45)⤻, there was a dramatic change in the extent of neuronal cell death in GM3 synthase-overexpressing embryos, especially in the CNS (mouse GM3 synthase: 66%, n=36; zebrafish GM3. Embryos younger than E were embedded in 1% agar before submission to minimize handling while orienting for paraffin embedding. For each time point, embryos were embedded on their backs, sides, or heads for sectioning in the respective frontal (coronal), sagittal (longitudinal), or .
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Ganglioside Composition of Normal and Mutant Mouse Embryos Ganglioside Composition of Normal and Mutant Mouse Embryos Bouvier, Joseph D.; Seyfried, Thomas N. Abstract: The enrichment of gangliosides in neuronal membranes suggests that they play an important role in CNS development.
We recently found a marked tetrasialoganglioside deficiency in tw1/tw1 mutant mouse embryos at embryonic. Address correspondence and reprint requests to Dr. Seyfried at Department of Biology, Boston College, Chestnut Hill, MAby: Ganglioside composition of normal and mutant mouse embryos.
Bouvier JD(1), Seyfried TN. Author information: (1)Department of Biology, Boston College, Chestnut Hill, Massachusetts The enrichment of gangliosides in neuronal membranes suggests that Cited by: The rate of GD3 formation was similar in whole embryos and in embryo head and body regions.
GD3 synthase activity int w1/t w1 mutant mouse embryos, which express defects in neuronal differentiation, was only Ganglioside composition of normal and mutant mouse embryos book of that in normal wild-type (+/+) embryos.
Enzyme activity in heterozygous (+/t w1) embryos was similar to that in +/+ by: 7. cogenesis. A better understanding of these ganglioside changes may provide clues as to the role of gangliosides in neural cell neoplasia.
Moreover, knowledge of tumor cell ganglioside com position can be useful for diagnostic and therapeutic purposes. Little is known about the ganglioside composition of exper imental mouse brain tumors.
Ganglioside composition of normal. and mutant mouse embryos. J Neurochem ; mental changes in ganglioside composition and synthesis in. The composition of whole embryo gangliosides at embryonic day E was compared among the C57BL/6 (B6) DBA/2 (D2), and C3H mouse strains.
N-acetylneuraminic acid was the predominant sialic acid species in the E embryos. N-glycolyneuraminic acid was either undetectable or present in only trace amounts.
Bouvier and Seyfried () observed that the predominant gangliosides in E12 mouse embryos were GD3 (51% of total sialic distribution), GM3 (19%), and GT1b (%); other gangliosides occurred in much lower amounts (GM2 (%), GM1 (%).
Bouvier and Seyfried () observed that the predominant gangliosides in E12 mouse embryos were GD3 (51% of total sialic distribution), GM3 (19%), and GT1b (%); other gangliosides occurred in. The ganglioside composition of these regions was the same as that of the whole embryo, with GM3 and GD3 comprising about 75% of the total ganglioside distribution.
Moreover, N -acetylneuraminic acid was the only sialic acid species detectable in the E and the E embryos. Various normal, spontaneously transformed and DNA tumor virus transformed mouse cell lines were grown in tissue culture and were analyzed for changes in ganglioside composition (cf 1,2).The cell lines included the highly contact inhibited mouse fibroblasts 3T3, its SV40 and polyoma doubly transformed variant SVPY 3T3; an established epithelial-like “normal” cell line from inbred mouse.
The content and composition of gangliosides were examined in an experimental mouse brain tumor, EPEN, that was grown subcutaneously in the flank of the syngeneic C57BL/6J (B6) host and in the B6 severe combined immunodeficiency (SCID) host.
SCID mice lack functional T- and B-lymphocytes, but have a normal complement of macrophages. The content and distribution of the brain tumor. Ganglioside GD3 biosynthesis in normal and mutant mouse embryos.
Biochemical Genetics29 (), DOI: /BF Laura Riboni, Guido Tettamanti. Rapid Internalization and Intracellular Metabolic Processing of Exogenous Ganglioside by. Alteration of Ganglioside Composition by Stable Transfection with Antisense Vectors against GD3-Synthase Gene Expression.
Influence of growth environment on the ganglioside composition of an experimental mouse brain tumor. Molecular and chemical Neutral glycolipid abnormalities in at-complex mutant mouse embryo.
Biochemical Genetics. The importance of regulated ganglioside expression is further underscored by the failed neuronal differentiation in mouse embryos 32 with a T-locus mutation which affects the synthesis of b-series gangliosides (Seyfried, ; Novikov and Seyfried, ) as well as in transgenic mouse embryos with a defect in the expression of 9-O-acetyl GD3, a.
Brain Research Bulletin, Vol. 24, pp. Pergamon Press plc, Printed in the U.S.A. /90 $ + REVIEW The Role(s) of Gangliosides in Neural Differentiation and Repair: A Perspective CARA-LYNNE SCHENGRUND Department of Biological Chemistry, The Milton S. Hershey Medical Center P.O.
BoxThe Pennsylvania State University, Hershey, PA. Gangliosides are a family of sialic acid-containing glycosphingolipids that are abundant in neurons and have a variety of functions in developing and mature tissues. We examined the expression of ganglioside GT1b in the embryonic preimplantation stage after freezing and thawing processes to determine the regulatory roles of ganglioside GT1b in early embryonic development.
ICR mouse embryos. Generation and analysis of mutant mice with defects in the pathways of ganglioside biosynthesis supported this model (Kolter et al., ; Sandhoff and Kolter, ).
Mutant mice defective in b-series gangliosides were viable and without apparent neurological abnormalities (Kawai et al., ; Okada et al., ) and reach an almost normal. T gene and alteration of ganglioside synthesis in mutant mice were described in a previous report (8).
The predicted changes in the ganglioside composition of the mutant mice are shown in Fig. Histological and Immunohistological Analysis. Weights of testes were examined sequentially and presented average weights from five mice in each group.
Bouvier et al. demonstrated that predominant gangliosides were GD3 (52%), GM3 (19%), and GT1b in the E mouse embryo, while b-series gangliosides, such as GD3, GD1b, GT1b, and GQ1b significantly reduced in the twl/twl mutant mouse.
Ganglioside GM3 and GD3 are markedly expressed in mid-embryonic brains, but their expression is significantly. A ganglioside is a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with one or more sialic acids (e.g. n-acetylneuraminic acid, NANA) linked on the sugaran acetylated derivative of the carbohydrate sialic acid, makes the head groups of gangliosides anionic at pH 7, which distinguishes them from globosides.
The name ganglioside was first applied by the.Bouvier JD, Seyfried TN () Ganglioside composition of normal and mutant mouse embryos. J Neurochem – CrossRef Google Scholar Cheresh DA, Pierschbacher MD, Herzig MA, Mujoo K () Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins.
Instead, many mutant embryos differ from wild-type embryos by cell-state composition. Adjacent bar plots reflect the median embryo composition. A reference key for our wild-type time series is.